TRNT1

TRNT1
Available structures
PDB	Ortholog search: PDBe RCSB
List of PDB id codes
	1OU5, 4X4W
Identifiers
Aliases	TRNT1, CCA1, MtCCA, CGI-47, SIFD, tRNA nucleotidyl transferase 1, RPEM
External IDs	OMIM: 612907; MGI: 1917297; HomoloGene: 9333; GeneCards: TRNT1; OMA:TRNT1 - orthologs
Gene location (Human)
Chr.	Chromosome 3 (human)
End	3,150,879 bp
Gene location (Mouse)
Chr.	Chromosome 6 (mouse)
End	106,759,435 bp
RNA expression pattern
	Top expressed in
	endothelial cell; ; secondary oocyte; ; gonad; ; Achilles tendon; ; parietal pleura; ; epithelium of colon; ; pancreatic epithelial cell; ; cartilage tissue; ; metanephros; ; testicle;
	Top expressed in
	spermatocyte; ; zygote; ; spermatid; ; genital tubercle; ; secondary oocyte; ; tail of embryo; ; yolk sac; ; ventricular zone; ; embryo; ; atrioventricular valve;
	More reference expression data
	n/a
Gene ontology
Molecular function	CTP:3'-cytidine-tRNA cytidylyltransferase activity; transferase activity; nucleotide binding; tRNA binding; nucleotidyltransferase activity; ATP binding; CTP:tRNA cytidylyltransferase activity; RNA binding; 5'-3' RNA polymerase activity; ATP:3'-cytidine-cytidine-tRNA adenylyltransferase activity;
Cellular component	mitochondrial matrix; intracellular anatomical structure; mitochondrion; nucleoplasm;
Biological process	RNA processing; tRNA 3'-terminal CCA addition; tRNA processing; tRNA 3'-end processing; mitochondrial tRNA 3'-end processing;
	Sources:Amigo / QuickGO
Orthologs
	51095
	70047
	ENSG00000072756
	ENSMUSG00000013736
	Q96Q11
	Q8K1J6
	NM_001302946; NM_016000; NM_182916
	NM_001242358; NM_001242360; NM_027296
	NP_001289875; NP_886552; NP_001354250; NP_001354251; NP_001354252
	NP_001229287; NP_001229289; NP_081572
	Wikidata
View/Edit Human	View/Edit Mouse

tRNA-nucleotidyltransferase 1, is an enzyme that in humans is encoded by the TRNT1 gene.⁵⁶⁷ This enzyme adds the nucleotide sequence CCA to the 3' end of tRNA, using ATP and CTP as substrates. The sequence creates the binding site for an amino acid.⁸

References

GRCh38: Ensembl release 89: ENSG00000072756 – Ensembl, May 2017
GRCm38: Ensembl release 89: ENSMUSG00000013736 – Ensembl, May 2017
"Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
"Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
Lai CH, Chou CY, Ch'ang LY, Liu CS, Lin W (Aug 2000). "Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics". Genome Res. 10 (5): 703–13. doi:10.1101/gr.10.5.703. PMC 310876. PMID 10810093.
Nagaike T, Suzuki T, Tomari Y, Takemoto-Hori C, Negayama F, Watanabe K, Ueda T (Oct 2001). "Identification and characterization of mammalian mitochondrial tRNA nucleotidyltransferases". J Biol Chem. 276 (43): 40041–9. doi:10.1074/jbc.M106202200. PMID 11504732.
"Entrez Gene: TRNT1 tRNA nucleotidyl transferase, CCA-adding, 1".
Lizano E, Scheibe M, Rammelt C, Betat H, Mörl M (May 2008). "A comparative analysis of CCA-adding enzymes from human and E. coli: differences in CCA addition and tRNA 3'-end repair". Biochimie. 90 (5): 762–72. doi:10.1016/j.biochi.2007.12.007. PMID 18226598.

Further reading

Hartley JL, Temple GF, Brasch MA (2001). "DNA cloning using in vitro site-specific recombination". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863.
Wiemann S, Weil B, Wellenreuther R, et al. (2001). "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs". Genome Res. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166.
Simpson JC, Wellenreuther R, Poustka A, et al. (2001). "Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing". EMBO Rep. 1 (3): 287–92. doi:10.1093/embo-reports/kvd058. PMC 1083732. PMID 11256614.
Reichert AS, Thurlow DL, Mörl M (2002). "A eubacterial origin for the human tRNA nucleotidyltransferase?". Biol. Chem. 382 (10): 1431–8. CiteSeerX 10.1.1.657.1235. doi:10.1515/BC.2001.176. PMID 11727826. S2CID 230616.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Augustin MA, Reichert AS, Betat H, et al. (2003). "Crystal structure of the human CCA-adding enzyme: insights into template-independent polymerization". J. Mol. Biol. 328 (5): 985–94. doi:10.1016/S0022-2836(03)00381-4. PMID 12729736.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
Wiemann S, Arlt D, Huber W, et al. (2004). "From ORFeome to biology: a functional genomics pipeline". Genome Res. 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC 528930. PMID 15489336.
Kimura K, Wakamatsu A, Suzuki Y, et al. (2006). "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes". Genome Res. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560.
Mehrle A, Rosenfelder H, Schupp I, et al. (2006). "The LIFEdb database in 2006". Nucleic Acids Res. 34 (Database issue): D415–8. doi:10.1093/nar/gkj139. PMC 1347501. PMID 16381901.
Lizano E, Schuster J, Müller M, et al. (2007). "A splice variant of the human CCA-adding enzyme with modified activity". J. Mol. Biol. 366 (4): 1258–65. doi:10.1016/j.jmb.2006.12.016. PMID 17204286.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000072756 – Ensembl, May 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000013736 – Ensembl, May 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid10810093-5] Lai CH, Chou CY, Ch'ang LY, Liu CS, Lin W (Aug 2000). "Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics". Genome Res. 10 (5): 703–13. doi:10.1101/gr.10.5.703. PMC 310876. PMID 10810093.

[pmid11504732-6] Nagaike T, Suzuki T, Tomari Y, Takemoto-Hori C, Negayama F, Watanabe K, Ueda T (Oct 2001). "Identification and characterization of mammalian mitochondrial tRNA nucleotidyltransferases". J Biol Chem. 276 (43): 40041–9. doi:10.1074/jbc.M106202200. PMID 11504732.

[entrez-7] "Entrez Gene: TRNT1 tRNA nucleotidyl transferase, CCA-adding, 1".

[8] Lizano E, Scheibe M, Rammelt C, Betat H, Mörl M (May 2008). "A comparative analysis of CCA-adding enzymes from human and E. coli: differences in CCA addition and tRNA 3'-end repair". Biochimie. 90 (5): 762–72. doi:10.1016/j.biochi.2007.12.007. PMID 18226598.

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