Substrata, plural of substratum, may refer to:
- Earth's substrata, the geologic layering of the Earth
- Hypokeimenon, sometimes translated as substratum, a concept in metaphysics
- Substrata (album), a 1997 ambient music album by Biosphere
- Substrata 2, a 2001 double album by Biosphere, including a remaster of the 1997 album
- Substrata (gardening), another term for subsoil
- Substrata (geology), layers of rock or sediment
- Substrata (linguistics), languages which influence another through linguistic contact