SPDEF

SPDEF
Available structures
PDB	Ortholog search: PDBe RCSB
List of PDB id codes
	2DKX
Identifiers
Aliases	SPDEF, PDEF, bA375E1.3, SAM pointed domain containing ETS transcription factor
External IDs	OMIM: 608144; MGI: 1353422; HomoloGene: 8231; GeneCards: SPDEF; OMA:SPDEF - orthologs
Gene location (Human)
Chr.	Chromosome 6 (human)
End	34,556,333 bp
Gene location (Mouse)
Chr.	Chromosome 17 (mouse)
End	27,947,929 bp
RNA expression pattern
	Top expressed in
	parotid gland; ; nasal epithelium; ; mucosa of transverse colon; ; prostate; ; mucosa of ileum; ; olfactory zone of nasal mucosa; ; buccal mucosa cell; ; trachea; ; mucosa of sigmoid colon; ; lactiferous duct;
	Top expressed in
	crypt of lieberkuhn of small intestine; ; gastric mucosa; ; epithelium of stomach; ; mucous cell of stomach; ; pyloric antrum; ; Paneth cell; ; parotid gland; ; duodenum; ; female urethra; ; seminal vesicula;
	More reference expression data
	; ; ; ;
	More reference expression data
Gene ontology
Molecular function	DNA-binding transcription factor activity; DNA binding; DNA-binding transcription repressor activity, RNA polymerase II-specific; sequence-specific DNA binding; DNA-binding transcription factor activity, RNA polymerase II-specific;
Cellular component	nucleus;
Biological process	multicellular organism development; cell differentiation; regulation of transcription, DNA-templated; negative regulation of transcription by RNA polymerase II; negative regulation of cell fate commitment; lung goblet cell differentiation; intestinal epithelial cell development; transcription, DNA-templated; positive regulation of cell fate commitment; positive regulation of transcription by RNA polymerase II; positive regulation of apoptotic process; regulation of transcription by RNA polymerase II;
	Sources:Amigo / QuickGO
Orthologs
	25803
	30051
	ENSG00000124664
	ENSMUSG00000024215
	O95238
	Q9WTP3
	NM_001252294; NM_012391
	NM_013891; NM_001357728
	NP_001239223; NP_036523
	NP_038919; NP_001344657
	Wikidata
View/Edit Human	View/Edit Mouse

SAM pointed domain-containing Ets transcription factor is a protein that in humans is encoded by the SPDEF gene.⁵⁶⁷

Originally named PDEF ⁸(Prostate derived ETS factor, also called PSE or prostate specific ETS ⁹), SPDEF is an ETS transcription factor expressed in prostate epithelial cells. In the gastrointestinal tract and lungs, it is expressed in mucus-secreting cells including intestinal and airway goblet cells, intestinal Paneth cells, gastric mucus gland and mucus neck cells, and Brunner's gland cells. ¹⁰¹¹¹² In the prostate, SPDEF acts as an androgen-independent transactivator of PSA (MIM 176820) expression.[supplied by OMIM]⁷

Interactions

SPDEF has been shown to interact with NKX3-1.¹³

References

GRCh38: Ensembl release 89: ENSG00000124664 – Ensembl, May 2017
GRCm38: Ensembl release 89: ENSMUSG00000024215 – Ensembl, May 2017
"Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
"Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
Oettgen P, Finger E, Sun Z, Akbarali Y, Thamrongsak U, Boltax J, et al. (January 2000). "PDEF, a novel prostate epithelium-specific ets transcription factor, interacts with the androgen receptor and activates prostate-specific antigen gene expression". The Journal of Biological Chemistry. 275 (2): 1216–1225. doi:10.1074/jbc.275.2.1216. PMID 10625666.
Johannessen JV, Finseth I (January 1983). "Electron micrographs in five minutes". Ultrastructural Pathology. 4 (1): 97–100. doi:10.3109/01913128309140576. PMID 6857767.
"Entrez Gene: SPDEF SAM pointed domain containing ets transcription factor".
Oettgen, P.; Finger, E.; Sun, Z.; Akbarali, Y.; Thamrongsak, U.; Boltax, J.; Grall, F.; Dube, A.; Weiss, A.; Brown, L.; Quinn, G.; Kas, K.; Endress, G.; Kunsch, C.; Libermann, T. A. (2000-01-14). "PDEF, a novel prostate epithelium-specific ets transcription factor, interacts with the androgen receptor and activates prostate-specific antigen gene expression". The Journal of Biological Chemistry. 275 (2): 1216–1225. doi:10.1074/jbc.275.2.1216. ISSN 0021-9258. PMID 10625666.
Yamada, N.; Tamai, Y.; Miyamoto, H.; Nozaki, M. (2000-01-11). "Cloning and expression of the mouse Pse gene encoding a novel Ets family member". Gene. 241 (2): 267–274. doi:10.1016/s0378-1119(99)00484-9. ISSN 0378-1119. PMID 10675039.
Horst, David; Gu, Xuesong; Bhasin, Manoj; Yang, Quanli; Verzi, Michael; Lin, Dongxu; Joseph, Marie; Zhang, Xiaobo; Chen, Wei; Li, Yi-Ping; Shivdasani, Ramesh A.; Libermann, Towia A. (2010-11-05). "Requirement of the epithelium-specific Ets transcription factor Spdef for mucous gland cell function in the gastric antrum". The Journal of Biological Chemistry. 285 (45): 35047–35055. doi:10.1074/jbc.M110.164541. ISSN 1083-351X. PMC 2966119. PMID 20801882.
Rajavelu, Priya; Chen, Gang; Xu, Yan; Kitzmiller, Joseph A.; Korfhagen, Thomas R.; Whitsett, Jeffrey A. (May 2015). "Airway epithelial SPDEF integrates goblet cell differentiation and pulmonary Th2 inflammation". The Journal of Clinical Investigation. 125 (5): 2021–2031. doi:10.1172/JCI79422. ISSN 1558-8238. PMC 4463206. PMID 25866971.
Noah, Taeko K.; Kazanjian, Avedis; Whitsett, Jeffrey; Shroyer, Noah F. (2010-02-01). "SAM pointed domain ETS factor (SPDEF) regulates terminal differentiation and maturation of intestinal goblet cells". Experimental Cell Research. 316 (3): 452–465. doi:10.1016/j.yexcr.2009.09.020. ISSN 1090-2422. PMC 3004755. PMID 19786015.
Chen H, Nandi AK, Li X, Bieberich CJ (January 2002). "NKX-3.1 interacts with prostate-derived Ets factor and regulates the activity of the PSA promoter". Cancer Research. 62 (2): 338–340. PMID 11809674.

Further reading

Lance V (1976). "Studies on the annual reproductive cycle of the female cobra, Naja naja. Seasonal variation in plasma inorganic ions". Comparative Biochemistry and Physiology. A, Comparative Physiology. 53 (3): 285–289. doi:10.1016/S0300-9629(76)80037-0. PMID 2437.
Yamada N, Tamai Y, Miyamoto H, Nozaki M (January 2000). "Cloning and expression of the mouse Pse gene encoding a novel Ets family member". Gene. 241 (2): 267–274. doi:10.1016/S0378-1119(99)00484-9. PMID 10675039.
Chen H, Nandi AK, Li X, Bieberich CJ (January 2002). "NKX-3.1 interacts with prostate-derived Ets factor and regulates the activity of the PSA promoter". Cancer Research. 62 (2): 338–340. PMID 11809674.
Thompson HG, Harris JW, Wold BJ, Lin F, Brody JP (April 2003). "p62 overexpression in breast tumors and regulation by prostate-derived Ets factor in breast cancer cells". Oncogene. 22 (15): 2322–2333. doi:10.1038/sj.onc.1206325. PMID 12700667.
Feldman RJ, Sementchenko VI, Gayed M, Fraig MM, Watson DK (August 2003). "Pdef expression in human breast cancer is correlated with invasive potential and altered gene expression". Cancer Research. 63 (15): 4626–4631. PMID 12907642.
Ghadersohi A, Odunsi K, Lele S, Collins Y, Greco WR, Winston J, et al. (February 2004). "Prostate derived Ets transcription factor shows better tumor-association than other cancer-associated molecules". Oncology Reports. 11 (2): 453–458. doi:10.3892/or.11.2.453. PMID 14719083.
Chen H, Bieberich CJ (January 2005). "Structural and functional analysis of domains mediating interaction between NKX-3.1 and PDEF". Journal of Cellular Biochemistry. 94 (1): 168–177. doi:10.1002/jcb.20297. PMID 15523673. S2CID 46494570.
Wang Y, Feng L, Said M, Balderman S, Fayazi Z, Liu Y, et al. (May 2005). "Analysis of the 2.0 A crystal structure of the protein-DNA complex of the human PDEF Ets domain bound to the prostate specific antigen regulatory site". Biochemistry. 44 (19): 7095–7106. doi:10.1021/bi047352t. PMID 15882048.
Fowler M, Borazanci E, McGhee L, Pylant SW, Williams BJ, Glass J, et al. (January 2006). "RUNX1 (AML-1) and RUNX2 (AML-3) cooperate with prostate-derived Ets factor to activate transcription from the PSA upstream regulatory region". Journal of Cellular Biochemistry. 97 (1): 1–17. doi:10.1002/jcb.20664. PMID 16237704. S2CID 20541109.
Gunawardane RN, Sgroi DC, Wrobel CN, Koh E, Daley GQ, Brugge JS (December 2005). "Novel role for PDEF in epithelial cell migration and invasion". Cancer Research. 65 (24): 11572–11580. doi:10.1158/0008-5472.CAN-05-1196. PMC 2919290. PMID 16357167.
Rodabaugh KJ, Mhawech-Fauceglia P, Groth J, Lele S, Sood AK (January 2007). "Prostate-derived Ets factor is overexpressed in serous epithelial ovarian tumors". International Journal of Gynecological Pathology. 26 (1): 10–15. doi:10.1097/01.pgp.0000225386.41244.bd. PMID 17197890. S2CID 25880565.
Park KS, Korfhagen TR, Bruno MD, Kitzmiller JA, Wan H, Wert SE, et al. (April 2007). "SPDEF regulates goblet cell hyperplasia in the airway epithelium". The Journal of Clinical Investigation. 117 (4): 978–988. doi:10.1172/JCI29176. PMC 1810569. PMID 17347682.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000124664 – Ensembl, May 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000024215 – Ensembl, May 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid10625666-5] Oettgen P, Finger E, Sun Z, Akbarali Y, Thamrongsak U, Boltax J, et al. (January 2000). "PDEF, a novel prostate epithelium-specific ets transcription factor, interacts with the androgen receptor and activates prostate-specific antigen gene expression". The Journal of Biological Chemistry. 275 (2): 1216–1225. doi:10.1074/jbc.275.2.1216. PMID 10625666.

[pmid6857767-6] Johannessen JV, Finseth I (January 1983). "Electron micrographs in five minutes". Ultrastructural Pathology. 4 (1): 97–100. doi:10.3109/01913128309140576. PMID 6857767.

[entrez-7] "Entrez Gene: SPDEF SAM pointed domain containing ets transcription factor".

[8] Oettgen, P.; Finger, E.; Sun, Z.; Akbarali, Y.; Thamrongsak, U.; Boltax, J.; Grall, F.; Dube, A.; Weiss, A.; Brown, L.; Quinn, G.; Kas, K.; Endress, G.; Kunsch, C.; Libermann, T. A. (2000-01-14). "PDEF, a novel prostate epithelium-specific ets transcription factor, interacts with the androgen receptor and activates prostate-specific antigen gene expression". The Journal of Biological Chemistry. 275 (2): 1216–1225. doi:10.1074/jbc.275.2.1216. ISSN 0021-9258. PMID 10625666.

[9] Yamada, N.; Tamai, Y.; Miyamoto, H.; Nozaki, M. (2000-01-11). "Cloning and expression of the mouse Pse gene encoding a novel Ets family member". Gene. 241 (2): 267–274. doi:10.1016/s0378-1119(99)00484-9. ISSN 0378-1119. PMID 10675039.

[10] Horst, David; Gu, Xuesong; Bhasin, Manoj; Yang, Quanli; Verzi, Michael; Lin, Dongxu; Joseph, Marie; Zhang, Xiaobo; Chen, Wei; Li, Yi-Ping; Shivdasani, Ramesh A.; Libermann, Towia A. (2010-11-05). "Requirement of the epithelium-specific Ets transcription factor Spdef for mucous gland cell function in the gastric antrum". The Journal of Biological Chemistry. 285 (45): 35047–35055. doi:10.1074/jbc.M110.164541. ISSN 1083-351X. PMC 2966119. PMID 20801882.

[11] Rajavelu, Priya; Chen, Gang; Xu, Yan; Kitzmiller, Joseph A.; Korfhagen, Thomas R.; Whitsett, Jeffrey A. (May 2015). "Airway epithelial SPDEF integrates goblet cell differentiation and pulmonary Th2 inflammation". The Journal of Clinical Investigation. 125 (5): 2021–2031. doi:10.1172/JCI79422. ISSN 1558-8238. PMC 4463206. PMID 25866971.

[12] Noah, Taeko K.; Kazanjian, Avedis; Whitsett, Jeffrey; Shroyer, Noah F. (2010-02-01). "SAM pointed domain ETS factor (SPDEF) regulates terminal differentiation and maturation of intestinal goblet cells". Experimental Cell Research. 316 (3): 452–465. doi:10.1016/j.yexcr.2009.09.020. ISSN 1090-2422. PMC 3004755. PMID 19786015.

[pmid11809674-13] Chen H, Nandi AK, Li X, Bieberich CJ (January 2002). "NKX-3.1 interacts with prostate-derived Ets factor and regulates the activity of the PSA promoter". Cancer Research. 62 (2): 338–340. PMID 11809674.

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