PRPF4

PRPF4
Available structures
PDB	Ortholog search: PDBe RCSB
List of PDB id codes
	1MZW, 3JCR
Identifiers
Aliases	PRPF4, HPRP4, HPRP4P, PRP4, Prp4p, RP70, SNRNP60, pre-mRNA processing factor 4
External IDs	OMIM: 607795; MGI: 1917302; HomoloGene: 3446; GeneCards: PRPF4; OMA:PRPF4 - orthologs
Gene location (Human)
Chr.	Chromosome 9 (human)
End	113,294,009 bp
Gene location (Mouse)
Chr.	Chromosome 4 (mouse)
End	62,345,227 bp
RNA expression pattern
	Top expressed in
	secondary oocyte; ; corpus epididymis; ; cartilage tissue; ; endothelial cell; ; islet of Langerhans; ; tail of epididymis; ; caput epididymis; ; embryo; ; Skeletal muscle tissue of biceps brachii; ; ventricular zone;
	Top expressed in
	hand; ; maxillary prominence; ; epiblast; ; mandibular prominence; ; foot; ; yolk sac; ; interventricular septum; ; primitive streak; ; cumulus cell; ; superior cervical ganglion;
	More reference expression data
	; ;
	More reference expression data
Gene ontology
Molecular function	U4 snRNA binding; protein binding; U6 snRNA binding;
Cellular component	Cajal body; nuclear speck; spliceosomal complex; spliceosomal snRNP complex; U4/U6 snRNP; nucleoplasm; U4/U6 x U5 tri-snRNP complex; nucleus; U2-type precatalytic spliceosome;
Biological process	RNA splicing, via transesterification reactions; mRNA processing; RNA processing; RNA splicing; mRNA splicing, via spliceosome;
	Sources:Amigo / QuickGO
Orthologs
	9128
	70052
	ENSG00000136875
	ENSMUSG00000066148
	O43172
	Q9DAW6
	NM_001244926; NM_004697; NM_001322266; NM_001322267
	NM_027297
	NP_001231855; NP_001309195; NP_001309196; NP_004688
	NP_081573
	Wikidata
View/Edit Human	View/Edit Mouse

U4/U6 small nuclear ribonucleoprotein Prp4 is a protein that is found in humans and encoded by the PRPF4 gene.⁵⁶⁷

The removal of introns from nuclear pre-mRNAs occurs on complexes called spliceosomes, which are made up of 4 small nuclear ribonucleoprotein (snRNP) particles and an undefined number of transiently associated splicing factors.

PRPF4 is 1 of several proteins that associates with U4 and U6 snRNPs.[supplied by OMIM]⁷

References

GRCh38: Ensembl release 89: ENSG00000136875 – Ensembl, May 2017
GRCm38: Ensembl release 89: ENSMUSG00000066148 – Ensembl, May 2017
"Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
"Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
Lauber J, Plessel G, Prehn S, Will CL, Fabrizio P, Groning K, Lane WS, Luhrmann R (Sep 1997). "The human U4/U6 snRNP contains 60 and 90kD proteins that are structurally homologous to the yeast splicing factors Prp4p and Prp3p". RNA. 3 (8): 926–41. PMC 1369537. PMID 9257651.
Horowitz DS, Kobayashi R, Krainer AR (Dec 1997). "A new cyclophilin and the human homologues of yeast Prp3 and Prp4 form a complex associated with U4/U6 snRNPs". RNA. 3 (12): 1374–87. PMC 1369579. PMID 9404889.
"Entrez Gene: PRPF4 PRP4 pre-mRNA processing factor 4 homolog (yeast)".

Further reading

Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
Horowitz DS, Krainer AR (1997). "A human protein required for the second step of pre-mRNA splicing is functionally related to a yeast splicing factor". Genes Dev. 11 (1): 139–51. doi:10.1101/gad.11.1.139. PMID 9000057.
Wang A, Forman-Kay J, Luo Y, et al. (1997). "Identification and characterization of human genes encoding Hprp3p and Hprp4p, interacting components of the spliceosome". Hum. Mol. Genet. 6 (12): 2117–26. doi:10.1093/hmg/6.12.2117. PMID 9328476.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
Heng HH, Wang A, Hu J (1998). "Mapping of the human HPRP3 and HPRP4 genes encoding U4/U6-associated splicing factors to chromosomes 1q21.1 and 9q31-q33". Genomics. 48 (2): 273–5. doi:10.1006/geno.1997.5181. PMID 9521884.
Kojima T, Zama T, Wada K, et al. (2001). "Cloning of human PRP4 reveals interaction with Clk1". J. Biol. Chem. 276 (34): 32247–56. doi:10.1074/jbc.M103790200. PMID 11418604.
Gonzalez-Santos JM, Wang A, Jones J, et al. (2002). "Central region of the human splicing factor Hprp3p interacts with Hprp4p". J. Biol. Chem. 277 (26): 23764–72. doi:10.1074/jbc.M111461200. PMID 11971898.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Reidt U, Wahl MC, Fasshauer D, et al. (2003). "Crystal structure of a complex between human spliceosomal cyclophilin H and a U4/U6 snRNP-60K peptide" (PDF). J. Mol. Biol. 331 (1): 45–56. doi:10.1016/S0022-2836(03)00684-3. hdl:11858/00-001M-0000-0012-F239-9. PMID 12875835.
Ingelfinger D, Göthel SF, Marahiel MA, et al. (2004). "Two protein-protein interaction sites on the spliceosome-associated human cyclophilin CypH". Nucleic Acids Res. 31 (16): 4791–6. doi:10.1093/nar/gkg660. PMC 169899. PMID 12907720.
Stanĕk D, Neugebauer KM (2004). "Detection of snRNP assembly intermediates in Cajal bodies by fluorescence resonance energy transfer". J. Cell Biol. 166 (7): 1015–25. doi:10.1083/jcb.200405160. PMC 2172029. PMID 15452143.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
Andersen JS, Lam YW, Leung AK, et al. (2005). "Nucleolar proteome dynamics". Nature. 433 (7021): 77–83. Bibcode:2005Natur.433...77A. doi:10.1038/nature03207. PMID 15635413. S2CID 4344740.
Barrios-Rodiles M, Brown KR, Ozdamar B, et al. (2005). "High-throughput mapping of a dynamic signaling network in mammalian cells". Science. 307 (5715): 1621–5. Bibcode:2005Sci...307.1621B. doi:10.1126/science.1105776. PMID 15761153. S2CID 39457788.
Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.
Liu S, Rauhut R, Vornlocher HP, Lührmann R (2006). "The network of protein-protein interactions within the human U4/U6.U5 tri-snRNP". RNA. 12 (7): 1418–30. doi:10.1261/rna.55406. PMC 1484429. PMID 16723661.
Huang B, Ahn YT, McPherson L, et al. (2007). "Interaction of PRP4 with Kruppel-like factor 13 regulates CCL5 transcription". J. Immunol. 178 (11): 7081–7. doi:10.4049/jimmunol.178.11.7081. PMC 2674583. PMID 17513757.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000136875 – Ensembl, May 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000066148 – Ensembl, May 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid9257651-5] Lauber J, Plessel G, Prehn S, Will CL, Fabrizio P, Groning K, Lane WS, Luhrmann R (Sep 1997). "The human U4/U6 snRNP contains 60 and 90kD proteins that are structurally homologous to the yeast splicing factors Prp4p and Prp3p". RNA. 3 (8): 926–41. PMC 1369537. PMID 9257651.

[pmid9404889-6] Horowitz DS, Kobayashi R, Krainer AR (Dec 1997). "A new cyclophilin and the human homologues of yeast Prp3 and Prp4 form a complex associated with U4/U6 snRNPs". RNA. 3 (12): 1374–87. PMC 1369579. PMID 9404889.

[entrez-7] "Entrez Gene: PRPF4 PRP4 pre-mRNA processing factor 4 homolog (yeast)".

1

2

3

4

5

6

7