Article · Wikipedia archive · Last revised Jun 6, 2026

NotI

NotI is a restriction enzyme isolated from the bacterium Nocardia otitidis.

Last revised
Jun 6, 2026
Read time
≈ 1 min
Length
40 w
Citations
2
Source
Wikipedia ↗

NotI is a restriction enzyme isolated from the bacterium Nocardia otitidis.1

Recognition sequence

Palindromic recognition sequence of NotI:1 

5'-GCGGCCGC-3'
3'-CGCCGGCG-5'

The ends generated by NotI digest: 

5'---GC     GGCCGC---3'
3'---CGCCGG     CG---5'
References

References

  1. Biolabs, New England. "NotI | NEB". www.neb.com. Retrieved 2016-05-08.