Monopolin is a protein complex that in budding yeast is composed of the four proteins CSM1, HRR25, LRS4, and MAM1. Monopolin is required for the segregation of homologous centromeres to opposite poles of a dividing cell during anaphase I of meiosis.1 This occurs by bridging DSN1 kinetochore proteins to sister kinetochores within the centromere to physically fuse them and allow for the microtubules to pull each homolog toward opposite mitotic spindles.2
Molecular structure
Monopolin is composed of a 4 CSM1:2 LRS4 complex which forms a V-shaped structure with two globular heads at the ends, which are responsible for directly crosslinking sister kinetochores.1 Bound to each CSM1 head is a MAM1 protein which recruits one copy of the HRR25 kinase.3 The hydrophobic cavity on the CSM1 subunit allows the hydrophobic regions of Monopolin receptor and kinetochore protein, DSN1, to bind to and fuse the sister kinetochores.2 Microtubules can then attach to the kinetochores on the homologous centromeres and pull them toward opposite mitotic spindles to complete anaphase of meiosis I.
References
References
- Corbett KD, Yip CK, Ee LS, Walz T, Amon A, Harrison SC (August 2010). "The monopolin complex crosslinks kinetochore components to regulate chromosome-microtubule attachments". Cell. 142 (4): 556–67. doi:10.1016/j.cell.2010.07.017. PMC 2955198. PMID 20723757.
- Plowman, Rebecca; Singh, Namit; Tromer, Eelco C.; Payan, Angel; Duro, Eris; Spanos, Christos; Rappsilber, Juri; Snel, Berend; Kops, Geert J. P.L.; Corbett, Kevin D.; Marston, Adele L. (2019-09-01). "The molecular basis of monopolin recruitment to the kinetochore". Chromosoma. 128 (3): 331–354. doi:10.1007/s00412-019-00700-0. ISSN 1432-0886. PMC 6823300. PMID 31037469.
- Corbett, Kevin D.; Harrison, Stephen C. (2012-06-28). "Molecular Architecture of the Yeast Monopolin Complex". Cell Reports. 1 (6): 583–589. doi:10.1016/j.celrep.2012.05.012. ISSN 2211-1247. PMC 3494995. PMID 22813733.