80 kDa MCM3-associated protein is a protein that in humans is encoded by the MCM3AP gene.56
Function
The minichromosome maintenance protein 3 (MCM3) is one of the MCM proteins essential for the initiation of DNA replication. The protein encoded by this gene is an MCM3 binding protein. It was reported to have phosphorylation-dependent DNA-primase activity, which was up-regulated in antigen immunization induced germinal center. This protein was demonstrated to be an acetyltransferase that acetylates MCM3 and plays a role in DNA replication. The mutagenesis of a nuclear localization signal of MCM3 affects the binding of this protein with MCM3, suggesting that this protein may also facilitate MCM3 nuclear localization.6
Interactions
MCM3AP has been shown to interact with:
References
References
- GRCh38: Ensembl release 89: ENSG00000160294 – Ensembl, May 2017
- GRCm38: Ensembl release 89: ENSMUSG00000001150 – Ensembl, May 2017
- "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- Takei Y, Tsujimoto G (September 1998). "Identification of a novel MCM3-associated protein that facilitates MCM3 nuclear localization". J. Biol. Chem. 273 (35): 22177–80. doi:10.1074/jbc.273.35.22177. PMID 9712829.
- "Entrez Gene: MCM3AP MCM3 minichromosome maintenance deficient 3 (S. cerevisiae) associated protein".
- Takei Y, Assenberg M, Tsujimoto G, Laskey R (November 2002). "The MCM3 acetylase MCM3AP inhibits initiation, but not elongation, of DNA replication via interaction with MCM3". J. Biol. Chem. 277 (45): 43121–5. doi:10.1074/jbc.C200442200. PMID 12226073.
- Kuwahara K, Yoshida M, Kondo E, Sakata A, Watanabe Y, Abe E, Kouno Y, Tomiyasu S, Fujimura S, Tokuhisa T, Kimura H, Ezaki T, Sakaguchi N (April 2000). "A novel nuclear phosphoprotein, GANP, is up-regulated in centrocytes of the germinal center and associated with MCM3, a protein essential for DNA replication". Blood. 95 (7): 2321–8. doi:10.1182/blood.V95.7.2321. PMID 10733502.
- Zhou Y, Li L, Liu Q, Xing G, Kuai X, Sun J, Yin X, Wang J, Zhang L, He F (May 2008). "E3 ubiquitin ligase SIAH1 mediates ubiquitination and degradation of TRB3". Cell. Signal. 20 (5): 942–8. doi:10.1016/j.cellsig.2008.01.010. PMID 18276110.
Further reading
Further reading
- Nagase T, Ishikawa K, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O (1998). "Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro". DNA Res. 5 (1): 31–9. doi:10.1093/dnares/5.1.31. PMID 9628581.
- Kuwahara K, Yoshida M, Kondo E, Sakata A, Watanabe Y, Abe E, Kouno Y, Tomiyasu S, Fujimura S, Tokuhisa T, Kimura H, Ezaki T, Sakaguchi N (2000). "A novel nuclear phosphoprotein, GANP, is up-regulated in centrocytes of the germinal center and associated with MCM3, a protein essential for DNA replication". Blood. 95 (7): 2321–8. doi:10.1182/blood.V95.7.2321. PMID 10733502.
- Abe E, Kuwahara K, Yoshida M, Suzuki M, Terasaki H, Matsuo Y, Takahashi EI, Sakaguchi N (2000). "Structure, expression, and chromosomal localization of the human gene encoding a germinal center-associated nuclear protein (GANP) that associates with MCM3 involved in the initiation of DNA replication". Gene. 255 (2): 219–27. doi:10.1016/S0378-1119(00)00336-X. PMID 11024281.
- Takei Y, Swietlik M, Tanoue A, Tsujimoto G, Kouzarides T, Laskey R (2001). "MCM3AP, a novel acetyltransferase that acetylates replication protein MCM3". EMBO Rep. 2 (2): 119–23. doi:10.1093/embo-reports/kve026. PMC 1083822. PMID 11258703.
- Kuwahara K, Tomiyasu S, Fujimura S, Nomura K, Xing Y, Nishiyama N, Ogawa M, Imajoh-Ohmi S, Izuta S, Sakaguchi N (2001). "Germinal center-associated nuclear protein (GANP) has a phosphorylation-dependent DNA-primase activity that is up-regulated in germinal center regions". Proc. Natl. Acad. Sci. U.S.A. 98 (18): 10279–83. Bibcode:2001PNAS...9810279K. doi:10.1073/pnas.181335698. PMC 56952. PMID 11526238.
- EL-Gazzar MA, Maeda K, Nomiyama H, Nakao M, Kuwahara K, Sakaguchi N (2001). "PU.1 is involved in the regulation of B lineage-associated and developmental stage-dependent expression of the germinal center-associated DNA primase GANP". J. Biol. Chem. 276 (51): 48000–8. doi:10.1074/jbc.M106696200. PMID 11641399.
- Kono Y, Maeda K, Kuwahara K, Yamamoto H, Miyamoto E, Yonezawa K, Takagi K, Sakaguchi N (2002). "MCM3-binding GANP DNA-primase is associated with a novel phosphatase component G5PR". Genes Cells. 7 (8): 821–34. doi:10.1046/j.1365-2443.2002.00562.x. PMID 12167160. S2CID 21946802.
- Takei Y, Assenberg M, Tsujimoto G, Laskey R (2002). "The MCM3 acetylase MCM3AP inhibits initiation, but not elongation, of DNA replication via interaction with MCM3". J. Biol. Chem. 277 (45): 43121–5. doi:10.1074/jbc.C200442200. PMID 12226073.
- Nakayama M, Kikuno R, Ohara O (2002). "Protein-protein interactions between large proteins: two-hybrid screening using a functionally classified library composed of long cDNAs". Genome Res. 12 (11): 1773–84. doi:10.1101/gr.406902. PMC 187542. PMID 12421765.
- Sakaguchi N, Fujimura S, Kuwahara K (2002). "Involvement of GANP in B cell activation in T cell-dependent antigen response". Dev. Immunol. 9 (3): 169–72. doi:10.1080/1044667031000137647. PMC 2276099. PMID 12885157.
- Colland F, Jacq X, Trouplin V, Mougin C, Groizeleau C, Hamburger A, Meil A, Wojcik J, Legrain P, Gauthier JM (2004). "Functional proteomics mapping of a human signaling pathway". Genome Res. 14 (7): 1324–32. doi:10.1101/gr.2334104. PMC 442148. PMID 15231748.
- Hu YH, Warnatz HJ, Vanhecke D, Wagner F, Fiebitz A, Thamm S, Kahlem P, Lehrach H, Yaspo ML, Janitz M (2006). "Cell array-based intracellular localization screening reveals novel functional features of human chromosome 21 proteins". BMC Genomics. 7 155. doi:10.1186/1471-2164-7-155. PMC 1526728. PMID 16780588.
External links
External links
- PDBe-KB provides an overview of all the structure information available in the PDB for Human Germinal-center associated nuclear protein (MCM3AP)
