| Imidazoleglycerol-phosphate dehydratase | |||||||
|---|---|---|---|---|---|---|---|
| Identifiers | |||||||
| Organism | |||||||
| Symbol | HIS3 | ||||||
| Entrez | 854377 | ||||||
| HomoloGene | 6979 | ||||||
| RefSeq (mRNA) | NM_001183621.1 | ||||||
| RefSeq (Prot) | NP_014845.1 | ||||||
| UniProt | P06633 | ||||||
| Other data | |||||||
| EC number | 4.2.1.19 | ||||||
| Chromosome | XV: 0.72 - 0.72 Mb | ||||||
| |||||||
The HIS3 gene, found in the Saccharomyces cerevisiae yeast, encodes a protein called Imidazoleglycerol-phosphate dehydratase which catalyses the sixth step in histidine biosynthesis.1 It is analogous to hisB in Escherichia coli.
Exploits
Mutations in Escherichia coli's analogous gene, hisB allows researchers to select only those individuals expressing the HIS3 gene included on a plasmid. The HIS3 gene is coupled to a certain promoter which can only be activated by successful binding of the relevant transcription factors. This is used in certain methods of bacterial two-hybrid screening to allow the survival of E. coli in which a desired protein-DNA or protein-protein interaction is taking place.2
References
References
- "HIS3/YOR202W Summary". Archived from the original on 2006-05-05. Retrieved 2006-11-30.
- Joung JK, Ramm EI, Pabo CO (June 2000). "A bacterial two-hybrid selection system for studying protein-DNA and protein-protein interactions". Proceedings of the National Academy of Sciences of the United States of America. 97 (13): 7382–7. Bibcode:2000PNAS...97.7382J. doi:10.1073/pnas.110149297. PMC 16554. PMID 10852947.