The human FUT8 gene encodes the enzyme Alpha-(1,6)-fucosyltransferase.56
This enzyme belongs to the family of fucosyltransferases. It catalyzes the transfer of fucose from GDP-fucose to N-linked type complex glycopeptides. This enzyme is distinct from other fucosyltransferases which catalyze alpha1-2, alpha1-3, and alpha1-4 fucose addition to existing oligosaccharides or the four Protein O-fucosyltransferases (POFUTs) that transfer fucose in alpha1-O linkage directly to serines or threonines in specific protein motifs. The expression of this gene may contribute to the malignancy of cancer cells and to their invasive and metastatic capabilities. Alternatively spliced variants encoding different isoforms have been identified.6
Kyowa Hakko Kirin's "Potelligent" platform uses a CHO cell line in which FUT8 has been knocked out to make afucosylated monoclonal antibodies.7
References
References
- GRCh38: Ensembl release 89: ENSG00000033170 – Ensembl, May 2017
- GRCm38: Ensembl release 89: ENSMUSG00000021065 – Ensembl, May 2017
- "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- Costache M, Apoil PA, Cailleau A, Elmgren A, Larson G, Henry S, et al. (November 1997). "Evolution of fucosyltransferase genes in vertebrates". The Journal of Biological Chemistry. 272 (47): 29721–29728. doi:10.1074/jbc.272.47.29721. PMID 9368041.
- "Entrez Gene: FUT8 fucosyltransferase 8 (alpha (1,6) fucosyltransferase)".
- Yu X, Marshall MJ, Cragg MS, Crispin M (June 2017). "Improving Antibody-Based Cancer Therapeutics Through Glycan Engineering". BioDrugs. 31 (3): 151–166. doi:10.1007/s40259-017-0223-8. PMID 28466278. S2CID 3722081.
Further reading
Further reading
- Miyoshi E, Noda K, Yamaguchi Y, Inoue S, Ikeda Y, Wang W, et al. (December 1999). "The alpha1-6-fucosyltransferase gene and its biological significance". Biochimica et Biophysica Acta (BBA) - General Subjects. 1473 (1): 9–20. doi:10.1016/s0304-4165(99)00166-x. PMID 10580126.
- Kalyanaraman VS, Rodriguez V, Veronese F, Rahman R, Lusso P, DeVico AL, et al. (March 1990). "Characterization of the secreted, native gp120 and gp160 of the human immunodeficiency virus type 1". AIDS Research and Human Retroviruses. 6 (3): 371–380. doi:10.1089/aid.1990.6.371. PMID 2187500.
- Pal R, Hoke GM, Sarngadharan MG (May 1989). "Role of oligosaccharides in the processing and maturation of envelope glycoproteins of human immunodeficiency virus type 1". Proceedings of the National Academy of Sciences of the United States of America. 86 (9): 3384–3388. Bibcode:1989PNAS...86.3384P. doi:10.1073/pnas.86.9.3384. PMC 287137. PMID 2541446.
- Dewar RL, Vasudevachari MB, Natarajan V, Salzman NP (June 1989). "Biosynthesis and processing of human immunodeficiency virus type 1 envelope glycoproteins: effects of monensin on glycosylation and transport". Journal of Virology. 63 (6): 2452–2456. doi:10.1128/jvi.63.6.2452-2456.1989. PMC 250699. PMID 2542563.
- Kozarsky K, Penman M, Basiripour L, Haseltine W, Sodroski J, Krieger M (1989). "Glycosylation and processing of the human immunodeficiency virus type 1 envelope protein". Journal of Acquired Immune Deficiency Syndromes. 2 (2): 163–169. PMID 2649653.
- Robinson WE, Montefiori DC, Mitchell WM (1988). "Evidence that mannosyl residues are involved in human immunodeficiency virus type 1 (HIV-1) pathogenesis". AIDS Research and Human Retroviruses. 3 (3): 265–282. doi:10.1089/aid.1987.3.265. PMID 2829950.
- Andersson B, Wentland MA, Ricafrente JY, Liu W, Gibbs RA (April 1996). "A "double adaptor" method for improved shotgun library construction". Analytical Biochemistry. 236 (1): 107–113. doi:10.1006/abio.1996.0138. PMID 8619474.
- Yu W, Andersson B, Worley KC, Muzny DM, Ding Y, Liu W, et al. (April 1997). "Large-scale concatenation cDNA sequencing". Genome Research. 7 (4): 353–358. doi:10.1101/gr.7.4.353. PMC 139146. PMID 9110174.
- Yanagidani S, Uozumi N, Ihara Y, Miyoshi E, Yamaguchi N, Taniguchi N (March 1997). "Purification and cDNA cloning of GDP-L-Fuc:N-acetyl-beta-D-glucosaminide:alpha1-6 fucosyltransferase (alpha1-6 FucT) from human gastric cancer MKN45 cells". Journal of Biochemistry. 121 (3): 626–632. doi:10.1093/oxfordjournals.jbchem.a021631. PMID 9133635.
- Yamaguchi Y, Fujii J, Inoue S, Uozumi N, Yanagidani S, Ikeda Y, et al. (1999). "Mapping of the alpha-1,6-fucosyltransferase gene, FUT8, to human chromosome 14q24.3". Cytogenetics and Cell Genetics. 84 (1–2): 58–60. doi:10.1159/000015215. PMID 10343104. S2CID 85305954.
- Takahashi T, Ikeda Y, Tateishi A, Yamaguchi Y, Ishikawa M, Taniguchi N (May 2000). "A sequence motif involved in the donor substrate binding by alpha1,6-fucosyltransferase: the role of the conserved arginine residues". Glycobiology. 10 (5): 503–510. doi:10.1093/glycob/10.5.503. PMID 10764839.
- Yamaguchi Y, Ikeda Y, Takahashi T, Ihara H, Tanaka T, Sasho C, et al. (June 2000). "Genomic structure and promoter analysis of the human alpha1, 6-fucosyltransferase gene (FUT8)". Glycobiology. 10 (6): 637–643. doi:10.1093/glycob/10.6.637. PMID 10814706.
- Takahashi T, Ikeda Y, Miyoshi E, Yaginuma Y, Ishikawa M, Taniguchi N (December 2000). "alpha1,6fucosyltransferase is highly and specifically expressed in human ovarian serous adenocarcinomas". International Journal of Cancer. 88 (6): 914–919. doi:10.1002/1097-0215(20001215)88:6<914::AID-IJC12>3.0.CO;2-1. PMID 11093814. S2CID 22110674.
- Roos C, Kolmer M, Mattila P, Renkonen R (February 2002). "Composition of Drosophila melanogaster proteome involved in fucosylated glycan metabolism". The Journal of Biological Chemistry. 277 (5): 3168–3175. doi:10.1074/jbc.M107927200. PMID 11698403.
- Coullin P, Crooijmans RP, Groenen MA, Heilig R, Mollicone R, Oriol R, et al. (2003). "Assignment of FUT8 to chicken chromosome band 5q1.4 and to human chromosome 14q23.2-->q24.1 by in situ hybridization. Conserved and compared synteny between human and chicken". Cytogenetic and Genome Research. 97 (3–4): 234–238. doi:10.1159/000066611. PMID 12438718. S2CID 27532607.
- Martinez-Duncker I, Michalski JC, Bauvy C, Candelier JJ, Mennesson B, Codogno P, et al. (January 2004). "Activity and tissue distribution of splice variants of alpha6-fucosyltransferase in human embryogenesis". Glycobiology. 14 (1): 13–25. doi:10.1093/glycob/cwh006. PMID 14514715.
- Ito Y, Miyauchi A, Yoshida H, Uruno T, Nakano K, Takamura Y, et al. (October 2003). "Expression of alpha1,6-fucosyltransferase (FUT8) in papillary carcinoma of the thyroid: its linkage to biological aggressiveness and anaplastic transformation". Cancer Letters. 200 (2): 167–172. doi:10.1016/S0304-3835(03)00383-5. PMID 14568171.