BAF agar

BAF agar or biotin-aneurin-folic acid agar is a type of agar growth medium containing peptones. It is used to grow cultures of mycorrhizal fungi.¹ It was first described by A.J.P. Oort in Nutritional requirements of Lactarius species and cultural characters in relation to taxonomy in 1981.¹ The acidic pH (5.8-6.3) of BAF agar inhibits bacterial growth.²

BAF agar typically contains:²

• 30.0 g/L glucose
• 2.0 g/L peptone
• 0.2 g/L yeast extract
• 0.5 g/L KH₂PO₄
• 0.5 g/L MgSO₄.7 H₂O
• 10.0 mg/L FeCl₃.6 H₂O
• 1.0 mg/L ZnSO₄.7 H₂O
• 5.0 mg/L MnSO₄
• 100.0 mg/L CaCl₂.2 H₂O
• 50.0 μg/L thiamine HCl
• 1.0 μg/L biotin
• 100.0 μg/L folic acid
• 50.0 μg/L inositol
• 15 g/L agar