Random hexamer

A random hexamer or random hexonucleotides are for various PCR applications such as rolling circle amplification to prime the DNA.

They are oligonucleotide sequences of 6 bases which are synthesised entirely randomly to give a numerous range of sequences that have the potential to anneal at many random points on a DNA sequence and act as a primer to commence first strand cDNA synthesis. ¹²³

References

"Reverse Transcription Applications - US".
"Primers for cDNA Synthesis". www.thermoscientificbio.com.
Hansen, KD; Brenner, SE; Dudoit, S (2010). "Biases in Illumina transcriptome sequencing caused by random hexamer priming". Nucleic Acids Res. 38 (12): e131. doi:10.1093/nar/gkq224. PMC 2896536. PMID 20395217.

[1] "Reverse Transcription Applications - US".

[2] "Primers for cDNA Synthesis". www.thermoscientificbio.com.

[3] Hansen, KD; Brenner, SE; Dudoit, S (2010). "Biases in Illumina transcriptome sequencing caused by random hexamer priming". Nucleic Acids Res. 38 (12): e131. doi:10.1093/nar/gkq224. PMC 2896536. PMID 20395217.

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