PFKFB2

PFKFB2
Identifiers
Aliases	PFKFB2, PFK-2/FBPase-2, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
External IDs	OMIM: 171835; MGI: 107815; HomoloGene: 88554; GeneCards: PFKFB2; OMA:PFKFB2 - orthologs
Gene location (Human)
Chr.	Chromosome 1 (human)
End	207,081,024 bp
Gene location (Mouse)
Chr.	Chromosome 1 (mouse)
End	130,656,990 bp
RNA expression pattern
	Top expressed in
	islet of Langerhans; ; oocyte; ; right lobe of thyroid gland; ; left lobe of thyroid gland; ; gastrocnemius muscle; ; rectum; ; apex of heart; ; duodenum; ; anterior pituitary; ; left ventricle;
	Top expressed in
	neural layer of retina; ; spermatocyte; ; islet of Langerhans; ; spermatid; ; superior frontal gyrus; ; pineal gland; ; lumbar subsegment of spinal cord; ; cerebellar cortex; ; dentate gyrus of hippocampal formation granule cell; ; primary visual cortex;
	More reference expression data
	; ;
	More reference expression data
Gene ontology
Molecular function	transferase activity; nucleotide binding; kinase activity; protein binding; catalytic activity; fructose-2,6-bisphosphate 2-phosphatase activity; hydrolase activity; ATP binding; protein kinase binding; 6-phosphofructo-2-kinase activity; kinase binding;
Cellular component	cytoplasm; cytosol; nucleus; nucleoplasm;
Biological process	lactate metabolic process; glycolytic process; phosphorylation; fructose metabolic process; response to glucose; positive regulation of glucokinase activity; fructose 2,6-bisphosphate metabolic process; pyruvate metabolic process; glucose catabolic process; metabolism; positive regulation of insulin secretion; dephosphorylation; carbohydrate phosphorylation; positive regulation of glycolytic process;
	Sources:Amigo / QuickGO
Orthologs
	5208
	18640
	ENSG00000123836
	ENSMUSG00000026409
	O60825
	P70265
	NM_001018053; NM_006212
	NM_001162415; NM_001162416; NM_008825; NM_001368843
	NP_001018063; NP_006203
	NP_001155887; NP_001155888; NP_032851; NP_001355772
	Wikidata
View/Edit Human	View/Edit Mouse

6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 is an enzyme that in humans is encoded by the PFKFB2 gene.⁵

The protein encoded by this gene is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. This protein regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for this gene.⁵

Interactions

PFKFB2 has been shown to interact with YWHAQ.⁶

References

GRCh38: Ensembl release 89: ENSG00000123836 – Ensembl, May 2017
GRCm38: Ensembl release 89: ENSMUSG00000026409 – Ensembl, May 2017
"Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
"Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
"Entrez Gene: PFKFB2 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2".
Pozuelo Rubio M, Peggie Mark, Wong Barry H C, Morrice Nick, MacKintosh Carol (Jul 2003). "14-3-3s regulate fructose-2,6-bisphosphate levels by binding to PKB-phosphorylated cardiac fructose-2,6-bisphosphate kinase/phosphatase". EMBO J. 22 (14). England: 3514–23. doi:10.1093/emboj/cdg363. ISSN 0261-4189. PMC 165633. PMID 12853467.

Further reading

Rider MH, Vandamme J, Lebeau E, et al. (1992). "The two forms of bovine heart 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase result from alternative splicing". Biochem. J. 285 (2): 405–11. doi:10.1042/bj2850405. PMC 1132803. PMID 1322130.
Hilliker CE, Darville MI, Aly MS, et al. (1991). "Human and rat chromosomal localization of two genes for 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase by analysis of somatic cell hybrids and in situ hybridization". Genomics. 10 (4): 867–73. doi:10.1016/0888-7543(91)90174-D. PMID 1655632.
Goldmuntz EA, Remmers EF, Zha H, et al. (1994). "Genetic map of seven polymorphic markers comprising a single linkage group on rat chromosome 5". Mamm. Genome. 4 (11): 670–5. doi:10.1007/BF00360905. PMID 7904197. S2CID 1892799.
Hirata T, Kato M, Okamura N, et al. (1998). "Expression of human placental-type 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase in various cells and cell lines". Biochem. Biophys. Res. Commun. 242 (3): 680–4. doi:10.1006/bbrc.1997.8024. PMID 9464277.
Heine-Suñer D, Díaz-Guillén MA, Lange AJ, Rodríguez de Córdoba S (1998). "Sequence and structure of the human 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase heart isoform gene (PFKFB2)". Eur. J. Biochem. 254 (1): 103–10. doi:10.1046/j.1432-1327.1998.2540103.x. PMID 9652401.
Hirata T, Watanabe M, Miura S, et al. (2001). "Inhibition of tumor cell growth by a specific 6-phosphofructo-2-kinase inhibitor, N-bromoacetylethanolamine phosphate, and its analogues". Biosci. Biotechnol. Biochem. 64 (10): 2047–52. doi:10.1271/bbb.64.2047. PMID 11129574. S2CID 38413244.
Kessler R, Eschrich K (2001). "Splice isoforms of ubiquitous 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase in human brain". Brain Res. Mol. Brain Res. 87 (2): 190–5. doi:10.1016/S0169-328X(01)00014-6. PMID 11245921.
Soejima H, Kawamoto S, Akai J, et al. (2001). "Isolation of novel heart-specific genes using the BodyMap database". Genomics. 74 (1): 115–20. doi:10.1006/geno.2001.6527. PMID 11374908.
Baltrusch S, Lenzen S, Okar DA, et al. (2001). "Characterization of glucokinase-binding protein epitopes by a phage-displayed peptide library. Identification of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase as a novel interaction partner". J. Biol. Chem. 276 (47): 43915–23. doi:10.1074/jbc.M105470200. PMID 11522786.
Marsin AS, Bouzin C, Bertrand L, Hue L (2002). "The stimulation of glycolysis by hypoxia in activated monocytes is mediated by AMP-activated protein kinase and inducible 6-phosphofructo-2-kinase". J. Biol. Chem. 277 (34): 30778–83. doi:10.1074/jbc.M205213200. hdl:2078.1/12913. PMID 12065600.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Pozuelo Rubio M, Peggie M, Wong BH, et al. (2003). "14-3-3s regulate fructose-2,6-bisphosphate levels by binding to PKB-phosphorylated cardiac fructose-2,6-bisphosphate kinase/phosphatase". EMBO J. 22 (14): 3514–23. doi:10.1093/emboj/cdg363. PMC 165633. PMID 12853467.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
Jin J, Smith FD, Stark C, et al. (2004). "Proteomic, functional, and domain-based analysis of in vivo 14-3-3 binding proteins involved in cytoskeletal regulation and cellular organization". Curr. Biol. 14 (16): 1436–50. Bibcode:2004CBio...14.1436J. doi:10.1016/j.cub.2004.07.051. PMID 15324660. S2CID 2371325.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
Gregory SG, Barlow KF, McLay KE, et al. (2006). "The DNA sequence and biological annotation of human chromosome 1". Nature. 441 (7091): 315–21. Bibcode:2006Natur.441..315G. doi:10.1038/nature04727. PMID 16710414.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000123836 – Ensembl, May 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000026409 – Ensembl, May 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[entrez-5] "Entrez Gene: PFKFB2 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2".

[pmid12853467-6] Pozuelo Rubio M, Peggie Mark, Wong Barry H C, Morrice Nick, MacKintosh Carol (Jul 2003). "14-3-3s regulate fructose-2,6-bisphosphate levels by binding to PKB-phosphorylated cardiac fructose-2,6-bisphosphate kinase/phosphatase". EMBO J. 22 (14). England: 3514–23. doi:10.1093/emboj/cdg363. ISSN 0261-4189. PMC 165633. PMID 12853467.

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