Article · Wikipedia archive · Last revised Jul 19, 2026

Optimized Multicolor Immunofluorescence Panel

Optimized Multicolor Immunofluorescence Panel, abbreviation OMIP, was a series of standardized multi-color flow cytometry panel launched by International Society for Advancement of Cytometry (ISAC), published in the form of columns in Cytometry Part A. The first two OMIP articles were published in September 2010 issue of this journal.

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Optimized Multicolor Immunofluorescence Panel, abbreviation OMIP, was a series of standardized multi-color flow cytometry panel launched by International Society for Advancement of Cytometry (ISAC), published in the form of columns in Cytometry Part A.123 The first two OMIP articles were published in September 2010 issue of this journal.4

Purpose

The goals of OMIPs are:

  1. to alleviate the development time for researchers who wish to use the same (or highly similar) panels
  2. to provide a starting point for the creation of novel OMIPs, and
  3. to provide a mechanism for attribution to the developers of the panel via citation of the publication.1

List

No. No. of colors Purpose Species Cell types Paper Year Cytometry
OMIP-001 16 T-cell cytokine production after in vitro stimulation and phenotyping of cytokine-producing T-cells Human PBMC 54 2010 BD LSR II
OMIP-002 16 Specificity and phenotype of CD8+ T-cells Human PBMC, bone marrow mononuclear cells 64 2010 BD LSR II
OMIP-003 12 Phenotyping memory B cells human PBMC, bone marrow, and tonsil mononuclear cell 7 2011 BD LSR II
OMIP-004 15 Determine Treg levels and to provide an in depth characterization of Tregs Human PBMC, ACK-lysed blood 8 2012 BD LSRFortessa
OMIP-005 11 T cell cytokine production and memory phenotype Rhesus macaque PBMC 9 2012 BD LSR II
OMIP-006 9 Enumerating and phenotyping of T regulatory cells and subsets Human PBMC 10 2012 BD LSR II
OMIP-007 14 Characterize the phenotype, maturation, and activation of NK cells Human PBMC 11 2012
OMIP-008 11 T cell cytokine production following in vitro stimulation Human PBMC, T cell clones/lines, tumor-resident T cell lines 12 2012
OMIP-009 10 phenotype and function of CD4+ and CD8+ T-cells Human PBMC 13 2012
OMIP-010 10 immunophenotyping of small hematopoietic cell Human Cerebrospinal fluid, bone marrow, peripheral blood 14 2012
OMIP-011 8 Characterization of CECs in peripheral blood Human Whole blood, RBC-lysed and washed 15 2012
OMIP-012 9 Human leukocyte reconstitution and phenotype Humanized mouse Whole blood, Ficoll-separated PBMC, splenocytes 16 2012
OMIP-013 13 T-cell differentiation Human PBMC 17 2012
OMIP-014 12 T-cell cytokine production and function after in vitro stimulation Human Cryopreserved PBMC 18 2012
OMIP-015 13 T-reg characterization and T-cell activation Human PBMC 19 2013
OMIP-016 10 Quality and phenotype of antigen-specific CD4 and CD8 T cells Cynomolgus macaque or Human Fresh or cryopreserved PBMCs 20 2013
OMIP-017 13 CD4+ helper T-cell subsets (Th1, Th2, Th9, Th17, Th17Th1, Th22 and TFH) Human PBMC 21 2013
OMIP-018 15 Phenotype of CD4 T cell subsets via chemokine receptor expression Human Fresh PBMC, Bone marrow mononuclear cells 2223 2013
OMIP-019 14 γδT-cells, iNKT-cells, haematopoietic precursors Human PBMC 24 2013
OMIP-020 12 γδ T-cell differentiation phenotype Human PBMC 25 2014
OMIP-021 14 NKT cells, γδ T-cells, MAIT cells, Memory T cell subsets Human PBMC 26 2014
OMIP-022 14 T-cell phenotyping, memory categorization, cytokine production, and function following in vitro stimulation Human Cryopreserved PBMC (adult and infant) 27 2014
OMIP-023 10 In-depth phenotyping of the main leukocyte subtypes in one run Human Fresh EDTA-treated peripheral blood, RBC-lysed and Fresh EDTA-treated Cord-blood 28 2014
OMIP-024 18 Characterize the phenotype of PBMC leukocyte subsets Human Cryopreserved PBMC from adults and infants 29 2014
OMIP-025 16 Characterization of antigen-specific T cells, TFH-like cells and NK cells Human Cryopreserved PBMC 30 2014
OMIP-026 14 Phenotype of B and plasma cells Rhesus macaque, African green monkey Fresh whole blood, PBMCs, mononuclear cells of bone marrow, lymph node, spleen, tonsil 31 2015
OMIP-027
OMIP-028
OMIP-029
OMIP-030
OMIP-031
OMIP-032 32
OMIP-033
OMIP-034
OMIP-035
OMIP-036
OMIP-037
OMIP-038
OMIP-039
OMIP-040
OMIP-041
OMIP-042
OMIP-043
OMIP-044
OMIP-045
OMIP-046
OMIP-047
OMIP-048
OMIP-049 19 Subset analysis of hematopoiesis and hematopoietic disease Human Any source containing human myeloid cells 33 2018 BD LSR II
OMIP-050 28 34
OMIP-051
OMIP-052
OMIP-053
OMIP-054
OMIP-055
OMIP-056
OMIP-057
OMIP-058
OMIP-059
OMIP-060
OMIP-061
OMIP-062 14 Myeloid and innate/adaptive lymphoid comprehensive immunophenotyping Human Whole peripheral blood, cord blood, PBMCs, nasal aspirate, and tracheal aspirate samples 35 2019 BD LSRFortessa
OMIP-063
OMIP-064
OMIP-065
OMIP-066
OMIP-067
OMIP-068
OMIP-069
OMIP-070
OMIP-071
OMIP-072
OMIP-073
OMIP-074
OMIP-075
OMIP-076
OMIP-077
OMIP-078
OMIP-079
OMIP-080
OMIP-081
OMIP-082 25
OMIP-083 18
OMIP-084 28
OMIP-085 8
OMIP-086 22
OMIP-087
OMIP-088
OMIP-089 8
OMIP-090 18
OMIP-091 27
OMIP-092 6
OMIP-093 41
OMIP-094 24
OMIP-095
OMIP-096
OMIP-097
OMIP-098
OMIP-099
OMIP-100
OMIP-101 27 Broad immunophenotyping of leukocytes including myeloid (neutrophils, monocytes, mDCs) and lymphoid cell lineages (conventional T cells, DURT cells, γδT cells, B cells, NK cells, ILCs) Human Fresh or fixed, cryopreserved whole blood 36 2024 BD FACSymphony A5
References

References

  1. Mahnke, Yolanda; Chattopadhyay, Pratip; Roederer, Mario (September 2010). "Publication of optimized multicolor immunofluorescence panels". Cytometry Part A. 77A (9): 814–818. doi:10.1002/cyto.a.20916. PMID 20722004.
  2. Tárnok, A (September 2016). "OMIPs start school". Cytometry Part A. 89 (9): 795–6. doi:10.1002/cyto.a.22976. PMID 27657547.
  3. Mahnke, Y; Tárnok, A (28 September 2020). "Celebrating 10 Years of OMIPs". Cytometry Part A. doi:10.1002/cyto.a.24233. PMID 32985101.
  4. Roederer, M; Tárnok, A (September 2010). "OMIPs--Orchestrating multiplexity in polychromatic science". Cytometry Part A. 77 (9): 811–2. doi:10.1002/cyto.a.20959. PMID 20722007.
  5. Mahnke, YD; Roederer, M (September 2010). "OMIP-001: Quality and phenotype of Ag-responsive human T-cells". Cytometry Part A. 77 (9): 819–20. doi:10.1002/cyto.a.20944. PMID 20722005.
  6. Chattopadhyay, PK; Roederer, M; Price, DA (September 2010). "OMIP-002: Phenotypic analysis of specific human CD8+ T-cells using peptide-MHC class I multimers for any of four epitopes". Cytometry Part A. 77 (9): 821–2. doi:10.1002/cyto.a.20945. PMID 20722006.
  7. Wei, C; Jung, J; Sanz, I (November 2011). "OMIP-003: phenotypic analysis of human memory B cells". Cytometry Part A. 79 (11): 894–6. doi:10.1002/cyto.a.21112. PMC 3199331. PMID 21796774.
  8. Biancotto, A; Dagur, PK; Fuchs, JC; Langweiler, M; McCoy JP, Jr (January 2012). "OMIP-004: in-depth characterization of human T regulatory cells". Cytometry Part A. 81 (1): 15–6. doi:10.1002/cyto.a.21158. PMC 3510978. PMID 22052639.
  9. Foulds, KE; Donaldson, M; Roederer, M (May 2012). "OMIP-005: Quality and phenotype of antigen-responsive rhesus macaque T cells". Cytometry Part A. 81 (5): 360–1. doi:10.1002/cyto.a.22008. PMC 11389872. PMID 22438313.
  10. Murdoch, DM; Staats, JS; Weinhold, KJ (April 2012). "OMIP-006: phenotypic subset analysis of human T regulatory cells via polychromatic flow cytometry". Cytometry Part A. 81 (4): 281–3. doi:10.1002/cyto.a.22024. PMC 3352600. PMID 22319016.
  11. Eller, MA; Currier, JR (June 2012). "OMIP-007: phenotypic analysis of human natural killer cells". Cytometry Part A. 81 (6): 447–9. doi:10.1002/cyto.a.22033. PMID 22411905.
  12. Zuleger, CL; Albertini, MR (June 2012). "OMIP-008: measurement of Th1 and Th2 cytokine polyfunctionality of human T cells". Cytometry Part A. 81 (6): 450–2. doi:10.1002/cyto.a.22035. PMC 3418807. PMID 22431369.
  13. Lamoreaux, L; Koup, RA; Roederer, M (May 2012). "OMIP-009: Characterization of antigen-specific human T-cells". Cytometry Part A. 81 (5): 362–3. doi:10.1002/cyto.a.22042. PMID 22438322.
  14. Preijers, FW; Huys, E; Moshaver, B (June 2012). "OMIP-010: a new 10-color monoclonal antibody panel for polychromatic immunophenotyping of small hematopoietic cell samples". Cytometry Part A. 81 (6): 453–5. doi:10.1002/cyto.a.22056. PMID 22532446.
  15. Lachmann, R; Lanuti, P; Miscia, S (July 2012). "OMIP-011: Characterization of circulating endothelial cells (CECs) in peripheral blood". Cytometry Part A. 81 (7): 549–51. doi:10.1002/cyto.a.22071. PMID 22648996.
  16. Long, BR; Stoddart, CA (August 2012). "OMIP-012: Phenotypic and numeric determination of human leukocyte reconstitution in humanized mice". Cytometry Part A. 81 (8): 646–8. doi:10.1002/cyto.a.22074. PMID 22653780.
  17. Mahnke, YD; Beddall, MH; Roederer, M (November 2012). "OMIP-013: differentiation of human T-cells". Cytometry Part A. 81 (11): 935–6. doi:10.1002/cyto.a.22201. PMID 23027685.
  18. De Rosa, SC; Carter, DK; McElrath, MJ (December 2012). "OMIP-014: validated multifunctional characterization of antigen-specific human T cells by intracellular cytokine staining". Cytometry Part A. 81 (12): 1019–21. doi:10.1002/cyto.a.22218. PMC 3581864. PMID 23081852.
  19. Mahnke, YD; Beddall, MH; Roederer, M (February 2013). "OMIP-015: human regulatory and activated T-cells without intracellular staining". Cytometry Part A. 83 (2): 179–81. doi:10.1002/cyto.a.22230. PMID 23166007.
  20. Guenounou, S; Bosquet, N; Dembek, CJ; Le Grand, R; Cosma, A (February 2013). "OMIP-016: Characterization of antigen-responsive macaque and human T-cells". Cytometry Part A. 83 (2): 182–4. doi:10.1002/cyto.a.22233. PMID 23184609.
  21. Mahnke, YD; Beddall, MH; Roederer, M (May 2013). "OMIP-017: human CD4(+) helper T-cell subsets including follicular helper cells". Cytometry Part A. 83 (5): 439–40. doi:10.1002/cyto.a.22269. PMC 4347402. PMID 23450796.
  22. Brodie, T; Brenna, E; Sallusto, F (June 2013). "OMIP-018: chemokine receptor expression on human T helper cells". Cytometry Part A. 83 (6): 530–2. doi:10.1002/cyto.a.22278. PMID 23504907.
  23. Brodie, Tess; Brenna, Elena; Sallusto, Federica (2013). "Corrigendum for OMIP-018: Chemokine receptor expression on human T helper cells". Cytometry Part A. 83 (11): 1041–1045. doi:10.1002/cyto.a.22346.
  24. Mahnke, YD; Beddall, MH; Roederer, M (August 2013). "OMIP-019: quantification of human γδT-cells, iNKT-cells, and hematopoietic precursors". Cytometry Part A. 83 (8): 676–8. doi:10.1002/cyto.a.22326. PMID 23897714.
  25. Wistuba-Hamprecht, K; Pawelec, G; Derhovanessian, E (June 2014). "OMIP-020: phenotypic characterization of human γδ T-cells by multicolor flow cytometry". Cytometry Part A. 85 (6): 522–4. doi:10.1002/cyto.a.22470. PMID 24756989.
  26. Gherardin, NA; Ritchie, DS; Godfrey, DI; Neeson, PJ (July 2014). "OMIP-021: Simultaneous quantification of human conventional and innate-like T-cell subsets". Cytometry Part A. 85 (7): 573–5. doi:10.1002/cyto.a.22475. PMID 24757011.
  27. Graves, AJ; Padilla, MG; Hokey, DA (July 2014). "OMIP-022: Comprehensive assessment of antigen-specific human T-cell functionality and memory". Cytometry Part A. 85 (7): 576–9. doi:10.1002/cyto.a.22478. PMC 4231567. PMID 24866990.
  28. Bocsi, J; Melzer, S; Dähnert, I; Tárnok, A (September 2014). "OMIP-023: 10-color, 13 antibody panel for in-depth phenotyping of human peripheral blood leukocytes". Cytometry Part A. 85 (9): 781–4. doi:10.1002/cyto.a.22505. PMID 25132115.
  29. Moncunill, G; Han, H; Dobaño, C; McElrath, MJ; De Rosa, SC (December 2014). "OMIP-024: pan-leukocyte immunophenotypic characterization of PBMC subsets in human samples". Cytometry Part A. 85 (12): 995–8. doi:10.1002/cyto.a.22580. PMC 4457440. PMID 25352070.
  30. Moncunill, G; Dobaño, C; McElrath, MJ; De Rosa, SC (April 2015). "OMIP-025: evaluation of human T- and NK-cell responses including memory and follicular helper phenotype by intracellular cytokine staining". Cytometry Part A. 87 (4): 289–92. doi:10.1002/cyto.a.22590. PMC 4454451. PMID 25407958.
  31. Neumann, B; Sopper, S; Stahl-Hennig, C (September 2015). "OMIP-026: Phenotypic analysis of B and plasma cells in rhesus macaques". Cytometry Part A. 87 (9): 800–2. doi:10.1002/cyto.a.22712. PMID 26115002.
  32. Unsworth, A; Anderson, R; Haynes, N; Britt, K (June 2016). "OMIP-032: Two multi-color immunophenotyping panels for assessing the innate and adaptive immune cells in the mouse mammary gland". Cytometry Part A. 89 (6): 527–30. doi:10.1002/cyto.a.22867. PMID 27214375.
  33. Zhu, G; Brayer, J; Padron, E; Mulé, JJ; Mailloux, AW (October 2018). "OMIP-049: Analysis of Human Myelopoiesis and Myeloid Neoplasms". Cytometry Part A. 93 (10): 982–986. doi:10.1002/cyto.a.23598. PMC 6484443. PMID 30347519.
  34. Nettey, L; Giles, AJ; Chattopadhyay, PK (November 2018). "OMIP-050: A 28-color/30-parameter Fluorescence Flow Cytometry Panel to Enumerate and Characterize Cells Expressing a Wide Array of Immune Checkpoint Molecules". Cytometry Part A. 93 (11): 1094–1096. doi:10.1002/cyto.a.23608. PMID 30347136.
  35. Swieboda, D; Guo, Y; Sagawe, S; Thwaites, RS; Nadel, S; Openshaw, PJM; Culley, FJ (December 2019). "OMIP-062: A 14-Color, 16-Antibody Panel for Immunophenotyping Human Innate Lymphoid, Myeloid and T Cells in Small Volumes of Whole Blood and Pediatric Airway Samples". Cytometry Part A. 95 (12): 1231–1235. doi:10.1002/cyto.a.23907. PMC 6972618. PMID 31633878.
  36. Imbratta, C; Reid, TD; Toefy, A; Scriba, TJ; Nemes, E (March 2024). "OMIP-101: 27-color flow cytometry panel for immunophenotyping of major leukocyte populations in fixed whole blood". Cytometry Part A. 105 (3): 165–170. doi:10.1002/cyto.a.24827. PMID 38343094.