GPAA1

GPAA1
Identifiers
Aliases	GPAA1, GAA1, hGAA1, glycosylphosphatidylinositol anchor attachment 1, GPIBD15
External IDs	OMIM: 603048; MGI: 1202392; HomoloGene: 37852; GeneCards: GPAA1; OMA:GPAA1 - orthologs
Gene location (Human)
Chr.	Chromosome 8 (human)
End	144,086,216 bp
Gene location (Mouse)
Chr.	Chromosome 15 (mouse)
End	76,219,107 bp
RNA expression pattern
	Top expressed in
	stromal cell of endometrium; ; right lobe of thyroid gland; ; apex of heart; ; left lobe of thyroid gland; ; right adrenal gland; ; left adrenal gland; ; right adrenal cortex; ; mucosa of transverse colon; ; left adrenal cortex; ; body of stomach;
	Top expressed in
	right kidney; ; lip; ; granulocyte; ; neural layer of retina; ; muscle of thigh; ; yolk sac; ; primary visual cortex; ; superior frontal gyrus; ; ventricular zone; ; proximal tubule;
	More reference expression data
	; ; ; ;
	More reference expression data
Gene ontology
Molecular function	tubulin binding; GPI-anchor transamidase activity; GPI anchor binding; protein binding;
Cellular component	integral component of membrane; endoplasmic reticulum membrane; GPI-anchor transamidase complex; endoplasmic reticulum; membrane;
Biological process	protein retention in ER lumen; GPI anchor biosynthetic process; attachment of GPI anchor to protein; protein-containing complex assembly;
	Sources:Amigo / QuickGO
Orthologs
	8733
	14731
	ENSG00000197858
	ENSMUSG00000022561
	O43292
	Q9WTK3
	NM_003801
	NM_010331
	NP_003792
	NP_034461
	Wikidata
View/Edit Human	View/Edit Mouse

Glycosylphosphatidylinositol anchor attachment 1 protein is a protein that in humans is encoded by the GPAA1 gene.⁵⁶

Posttranslational glycosylphosphatidylinositol (GPI) anchor attachment serves as a general mechanism for linking proteins to the cell surface membrane. The protein encoded by this gene presumably functions in GPI anchoring at the GPI transfer step. The mRNA transcript is ubiquitously expressed in both fetal and adult tissues. The anchor attachment protein 1 contains an N-terminal signal sequence, 1 cAMP- and cGMP-dependent protein kinase phosphorylation site, 1 leucine zipper pattern, 2 potential N-glycosylation sites, and 8 putative transmembrane domains.⁶

Interactions

GPAA1 has been shown to interact with PIGT⁷⁸ and PIGK.⁸⁹

References

GRCh38: Ensembl release 89: ENSG00000197858 – Ensembl, May 2017
GRCm38: Ensembl release 89: ENSMUSG00000022561 – Ensembl, May 2017
"Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
"Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
Hiroi Y, Komuro I, Matsushita I, Aburatani H, Hosoda T, Nakahori Y, et al. (December 1998). "Assignment of the human GPAA1 gene, which encodes a product required for the attachment of glycosylphosphatidylinositols to proteins, at 8q24". Genomics. 54 (2): 354–5. doi:10.1006/geno.1998.5490. PMID 9828142.
"Entrez Gene: GPAA1 glycosylphosphatidylinositol anchor attachment protein 1 homolog (yeast)".
Ohishi K, Inoue N, Kinoshita T (August 2001). "PIG-S and PIG-T, essential for GPI anchor attachment to proteins, form a complex with GAA1 and GPI8". The EMBO Journal. 20 (15): 4088–98. doi:10.1093/emboj/20.15.4088. PMC 149153. PMID 11483512.
Vainauskas S, Maeda Y, Kurniawan H, Kinoshita T, Menon AK (August 2002). "Structural requirements for the recruitment of Gaa1 into a functional glycosylphosphatidylinositol transamidase complex". The Journal of Biological Chemistry. 277 (34): 30535–42. doi:10.1074/jbc.M205402200. PMID 12052837.
Ohishi K, Inoue N, Maeda Y, Takeda J, Riezman H, Kinoshita T (May 2000). "Gaa1p and gpi8p are components of a glycosylphosphatidylinositol (GPI) transamidase that mediates attachment of GPI to proteins". Molecular Biology of the Cell. 11 (5): 1523–33. doi:10.1091/mbc.11.5.1523. PMC 14864. PMID 10793132.

Further reading

Hiroi Y, Komuro I, Chen R, Hosoda T, Mizuno T, Kudoh S, et al. (January 1998). "Molecular cloning of human homolog of yeast GAA1 which is required for attachment of glycosylphosphatidylinositols to proteins". FEBS Letters. 421 (3): 252–8. doi:10.1016/S0014-5793(97)01576-7. PMID 9468317. S2CID 30327972.
Inoue N, Ohishi K, Endo Y, Fujita T, Takeda J, Kinoshita T (1999). "Human and mouse GPAA1 (Glycosylphosphatidylinositol anchor attachment 1) genes: genomic structures, chromosome loci and the presence of a minor class intron". Cytogenetics and Cell Genetics. 84 (3–4): 199–205. doi:10.1159/000015258. PMID 10393431. S2CID 23878595.
Ohishi K, Inoue N, Maeda Y, Takeda J, Riezman H, Kinoshita T (May 2000). "Gaa1p and gpi8p are components of a glycosylphosphatidylinositol (GPI) transamidase that mediates attachment of GPI to proteins". Molecular Biology of the Cell. 11 (5): 1523–33. doi:10.1091/mbc.11.5.1523. PMC 14864. PMID 10793132.
Ohishi K, Inoue N, Kinoshita T (August 2001). "PIG-S and PIG-T, essential for GPI anchor attachment to proteins, form a complex with GAA1 and GPI8". The EMBO Journal. 20 (15): 4088–98. doi:10.1093/emboj/20.15.4088. PMC 149153. PMID 11483512.
Vainauskas S, Maeda Y, Kurniawan H, Kinoshita T, Menon AK (August 2002). "Structural requirements for the recruitment of Gaa1 into a functional glycosylphosphatidylinositol transamidase complex". The Journal of Biological Chemistry. 277 (34): 30535–42. doi:10.1074/jbc.M205402200. PMID 12052837.
Ohishi K, Nagamune K, Maeda Y, Kinoshita T (April 2003). "Two subunits of glycosylphosphatidylinositol transamidase, GPI8 and PIG-T, form a functionally important intermolecular disulfide bridge". The Journal of Biological Chemistry. 278 (16): 13959–67. doi:10.1074/jbc.M300586200. PMID 12582175.
Hong Y, Ohishi K, Kang JY, Tanaka S, Inoue N, Nishimura J, et al. (May 2003). "Human PIG-U and yeast Cdc91p are the fifth subunit of GPI transamidase that attaches GPI-anchors to proteins". Molecular Biology of the Cell. 14 (5): 1780–9. doi:10.1091/mbc.E02-12-0794. PMC 165076. PMID 12802054.
Vainauskas S, Menon AK (February 2004). "A conserved proline in the last transmembrane segment of Gaa1 is required for glycosylphosphatidylinositol (GPI) recognition by GPI transamidase". The Journal of Biological Chemistry. 279 (8): 6540–5. doi:10.1074/jbc.M312191200. PMID 14660601.
Vainauskas S, Menon AK (April 2005). "Endoplasmic reticulum localization of Gaa1 and PIG-T, subunits of the glycosylphosphatidylinositol transamidase complex". The Journal of Biological Chemistry. 280 (16): 16402–9. doi:10.1074/jbc.M414253200. PMID 15713669.
Ho JC, Cheung ST, Patil M, Chen X, Fan ST (September 2006). "Increased expression of glycosyl-phosphatidylinositol anchor attachment protein 1 (GPAA1) is associated with gene amplification in hepatocellular carcinoma". International Journal of Cancer. 119 (6): 1330–7. doi:10.1002/ijc.22005. PMID 16642471. S2CID 33616131.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000197858 – Ensembl, May 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000022561 – Ensembl, May 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid9828142-5] Hiroi Y, Komuro I, Matsushita I, Aburatani H, Hosoda T, Nakahori Y, et al. (December 1998). "Assignment of the human GPAA1 gene, which encodes a product required for the attachment of glycosylphosphatidylinositols to proteins, at 8q24". Genomics. 54 (2): 354–5. doi:10.1006/geno.1998.5490. PMID 9828142.

[entrez-6] "Entrez Gene: GPAA1 glycosylphosphatidylinositol anchor attachment protein 1 homolog (yeast)".

[pmid11483512-7] Ohishi K, Inoue N, Kinoshita T (August 2001). "PIG-S and PIG-T, essential for GPI anchor attachment to proteins, form a complex with GAA1 and GPI8". The EMBO Journal. 20 (15): 4088–98. doi:10.1093/emboj/20.15.4088. PMC 149153. PMID 11483512.

[pmid12052837-8] Vainauskas S, Maeda Y, Kurniawan H, Kinoshita T, Menon AK (August 2002). "Structural requirements for the recruitment of Gaa1 into a functional glycosylphosphatidylinositol transamidase complex". The Journal of Biological Chemistry. 277 (34): 30535–42. doi:10.1074/jbc.M205402200. PMID 12052837.

[pmid10793132-9] Ohishi K, Inoue N, Maeda Y, Takeda J, Riezman H, Kinoshita T (May 2000). "Gaa1p and gpi8p are components of a glycosylphosphatidylinositol (GPI) transamidase that mediates attachment of GPI to proteins". Molecular Biology of the Cell. 11 (5): 1523–33. doi:10.1091/mbc.11.5.1523. PMC 14864. PMID 10793132.

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