DAD1

DAD1
Identifiers
Aliases	DAD1, OST2, defender against cell death 1
External IDs	OMIM: 600243; MGI: 101912; HomoloGene: 1027; GeneCards: DAD1; OMA:DAD1 - orthologs
Gene location (Human)
Chr.	Chromosome 14 (human)
End	22,589,224 bp
Gene location (Mouse)
Chr.	Chromosome 14 (mouse)
End	54,491,561 bp
RNA expression pattern
	Top expressed in
	islet of Langerhans; ; gallbladder; ; right lobe of thyroid gland; ; anterior pituitary; ; left lobe of thyroid gland; ; right adrenal gland; ; canal of the cervix; ; rectum; ; left adrenal gland; ; stromal cell of endometrium;
	Top expressed in
	choroid plexus of fourth ventricle; ; facial motor nucleus; ; seminal vesicula; ; islet of Langerhans; ; fossa; ; internal carotid artery; ; efferent ductule; ; adrenal gland; ; external carotid artery; ; carotid body;
	More reference expression data
	More reference expression data
Gene ontology
Molecular function	dolichyl-diphosphooligosaccharide-protein glycotransferase activity; oligosaccharyl transferase activity;
Cellular component	integral component of membrane; oligosaccharyltransferase complex; extracellular exosome; endoplasmic reticulum membrane; membrane; endoplasmic reticulum;
Biological process	protein glycosylation; negative regulation of apoptotic process; protein N-linked glycosylation via asparagine; blastocyst development; response to nutrient; apoptotic process; protein N-linked glycosylation;
	Sources:Amigo / QuickGO
Orthologs
	1603
	13135
	ENSG00000129562
	ENSMUSG00000022174
	P61803
	P61804
	NM_001344
	NM_001113358; NM_010015
	NP_001335
	NP_001106829; NP_034145
	Wikidata
View/Edit Human	View/Edit Mouse

Dolichyl-diphosphooligosaccharide—protein glycosyltransferase subunit DAD1 is an enzyme that in humans is encoded by the DAD1 gene.⁵

Function

DAD1, the defender against apoptotic cell death, was initially identified as a negative regulator of programmed cell death in the temperature sensitive tsBN7 cell line. The DAD1 protein disappeared in temperature-sensitive cells following a shift to the nonpermissive temperature, suggesting that loss of the DAD1 protein triggered apoptosis. DAD1 is believed to be a tightly associated subunit of oligosaccharyltransferase both in the intact membrane and in the purified enzyme, thus reflecting the essential nature of N-linked glycosylation in eukaryotes.⁵

Interactions

DAD1 has been shown to interact with MCL1.⁶

References

GRCh38: Ensembl release 89: ENSG00000129562 – Ensembl, May 2017
GRCm38: Ensembl release 89: ENSMUSG00000022174 – Ensembl, May 2017
"Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
"Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
"Entrez Gene: DAD1 defender against cell death 1".
Makishima T, Yoshimi M, Komiyama S, Hara N, Nishimoto T (September 2000). "A subunit of the mammalian oligosaccharyltransferase, DAD1, interacts with Mcl-1, one of the bcl-2 protein family". J. Biochem. 128 (3): 399–405. doi:10.1093/oxfordjournals.jbchem.a022767. PMID 10965038.

Further reading

Yulug IG, See CG, Fisher EM, Ylug IG (1995). "The DAD1 protein, whose defect causes apoptotic cell death, maps to human chromosome 14". Genomics. 26 (2): 433–5. doi:10.1016/0888-7543(95)80241-D. PMID 7601483.
Apte SS, Mattei MG, Seldin MF, Olsen BR (1995). "The highly conserved defender against the death 1 (DAD1) gene maps to human chromosome 14q11-q12 and mouse chromosome 14 and has plant and nematode homologs". FEBS Lett. 363 (3): 304–6. doi:10.1016/0014-5793(95)00321-Y. PMID 7737422. S2CID 39270530.
Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
Nakashima T, Sekiguchi T, Kuraoka A, Fukushima K, Shibata Y, Komiyama S, Nishimoto T (1993). "Molecular cloning of a human cDNA encoding a novel protein, DAD1, whose defect causes apoptotic cell death in hamster BHK21 cells". Mol. Cell. Biol. 13 (10): 6367–74. doi:10.1128/mcb.13.10.6367. PMC 364695. PMID 8413235.
Kelleher DJ, Gilmore R (1997). "DAD1, the defender against apoptotic cell death, is a subunit of the mammalian oligosaccharyltransferase". Proc. Natl. Acad. Sci. U.S.A. 94 (10): 4994–9. Bibcode:1997PNAS...94.4994K. doi:10.1073/pnas.94.10.4994. PMC 24619. PMID 9144178.
Makishima T, Nakashima T, Nagata-Kuno K, Fukushima K, Iida H, Sakaguchi M, Ikehara Y, Komiyama S, Nishimoto T (1997). "The highly conserved DAD1 protein involved in apoptosis is required for N-linked glycosylation". Genes Cells. 2 (2): 129–41. doi:10.1046/j.1365-2443.1997.1070303.x. PMID 9167970. S2CID 25807010.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
Zhong XP, Krangel MS (1999). "Enhancer-blocking activity within the DNase I hypersensitive site 2 to 6 region between the TCR alpha and Dad1 genes". J. Immunol. 163 (1): 295–300. doi:10.4049/jimmunol.163.1.295. PMID 10384128.
Makishima T, Yoshimi M, Komiyama S, Hara N, Nishimoto T (2000). "A subunit of the mammalian oligosaccharyltransferase, DAD1, interacts with Mcl-1, one of the bcl-2 protein family". J. Biochem. 128 (3): 399–405. doi:10.1093/oxfordjournals.jbchem.a022767. PMID 10965038.
Gevaert K, Goethals M, Martens L, Van Damme J, Staes A, Thomas GR, Vandekerckhove J (2003). "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides". Nat. Biotechnol. 21 (5): 566–9. doi:10.1038/nbt810. PMID 12665801. S2CID 23783563.
Shibatani T, David LL, McCormack AL, Frueh K, Skach WR (2005). "Proteomic analysis of mammalian oligosaccharyltransferase reveals multiple subcomplexes that contain Sec61, TRAP, and two potential new subunits". Biochemistry. 44 (16): 5982–92. doi:10.1021/bi047328f. PMID 15835887.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000129562 – Ensembl, May 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000022174 – Ensembl, May 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[entrez-5] "Entrez Gene: DAD1 defender against cell death 1".

[pmid10965038-6] Makishima T, Yoshimi M, Komiyama S, Hara N, Nishimoto T (September 2000). "A subunit of the mammalian oligosaccharyltransferase, DAD1, interacts with Mcl-1, one of the bcl-2 protein family". J. Biochem. 128 (3): 399–405. doi:10.1093/oxfordjournals.jbchem.a022767. PMID 10965038.

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